eDNA detection of invasive insects

Biosecurity outcome

• The suitability of eDNA for biosecurity surveillance will be assessed (proof-of-concept) using a pentatomid/brown marmorated stink bug model system. This will include the establishment of baseline information on eDNA detection of BMSB in NZ and comparison of eDNA detection with current technologies, with the ultimate aim of developing a BMSB eDNA trapping network.

Science outcome

• New approaches for eDNA preparation and amplification from terrestrial surfaces will be tested and reported.  “Simple” steps such as measuring and quantifying the detection limits for insect eDNA in the terrestrial environment will represent important scientific advances.
• The limits of detection for pentatomid/Brown Marmorated Stink Bug (BMSB) eDNA from environmental substrates will be quantified. Working with MPI, Port of Tauranga (PoT) and grower groups.

Key end users/stakeholders

Implementation:  Proof of concept.  Implementation >5 years.  Development of concept will be reported through SPF, KVH (co-funder), GIA and MPI (PHEL and Surveillance).  At appropriate time, co-funder and those responsible for biosecurity surveillance (i.e. GIA/MPI/) will be approached to develop pathway for implementation.